per litre

10x Transfer buffer

 

Trizma® base

25mM * 10 = 250mM = 0.250 * 121.1 = 30.38g

Glycine

192mM * 10 = 1.92M = 1.92 * 75.07 = 144.1g

 

 

1x Transfer buffer

 

10x Transfer buffer

100ml

Methanol

200ml

dH2O

700ml

 

 

10x TBS pH 7.6 (adjust with HCl)

 

Trizma® base

20mM * 10 = 200mM = 0.200 * 121.1 = 24.22g

NaCl

137mM * 10 = 1.37M = 1.37 * 58.44 = 80.06g

 

 

5% Blocking buffer pH 7.5

 

1xTBS

1000ml

5% dry milk (Semper)

25g

0.1% Tween®20

1ml

 

 

Staining solution

 

Coomassie Brilliant Blue

2ml

Methanol

400ml

Acetic acid

70ml

dH2O

528ml

 

 

Destaining solution

 

Methanol

400ml

Acetic acid

70ml

dH2O

530ml

 

 

Swedish chocolate cake

 

Eggs

 2

Butter

 150g

Sugar

 3dl (1.3 cups)

Flour

 1.5dl (0.6 cups)

Cocoa powder

 4msk (4 table spoons)

Vanilla sugar

 2tsk (2 tea spoons)

Salt

 a pinch

Bake in the oven for 150ºC, 23min.

 

 
 
…what? Chocolate cake?
 
Well, it may not be vital for the Western blot, but hey, it is just *brilliant* for late study nights!